NFE2 antibody (C-Term)

Catalog No. ABIN2777229

The Rabbit Polyclonal anti-NFE2 antibody is suitable to detect NFE2 in samples from Human, Mouse, Rat, Horse, Cow, Guinea Pig, Dog and Rabbit. It has been validated for WB.

Quick Overview for NFE2 antibody (C-Term) (ABIN2777229)

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Reactivity: Human, Mouse, Rat, Horse, Cow, Guinea Pig, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFE2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NFE2 Antibody

Binding Specificity: C-Term

Purpose: Nfe2 Antibody - C-terminal region

Sequence: ARGEADRTLE VMRQQLAELY HDIFQHLRDE SGNSYSPEEY VLQQAADGAI

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 93%, Horse: 79%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against Nfe2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of MOUSE Nfe2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP31216-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFE2

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Alternative Name: Nfe2

Background:

Background Information: Nfe2 is a component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation.It binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. It requires MAFK or other small MAF proteins for binding to the NF-E2 motif. It may play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

Gene Name: nuclear factor (erythroid-derived 2), 45 kDa

Alternative Symbols: p4, p45, NF-E, NF-E2, p45NF, p45nf-, p45NFE2, p45nf-e2, NF-E2/P45

Protein Name: Transcription factor NF-E2 45 kDa subunit

Molecular Weight: 41kDa

Gene ID: 18022

NCBI Accession: NP_032711

UniProt: Q07279