NFE2 antibody (N-Term)

Catalog No. ABIN2777230

The Rabbit Polyclonal anti-NFE2 antibody (ABIN2777230) specifically detects NFE2 in WB. The antibody is reactive with Human, Mouse, Rat, Horse, Cow and Guinea Pig samples.

Quick Overview for NFE2 antibody (N-Term) (ABIN2777230)

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Reactivity: Human, Mouse, Rat, Horse, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFE2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NFE2 Antibody

Binding Specificity: N-Term

Purpose: NFE2 Antibody - N-terminal region

Sequence: MSPCPPQQSR NRVIQLSTSE LGEMELTWQE IMSITELQGL NAPSEPSFEP

Predicted Reactivity: Cow: 92%, Guinea Pig: 87%, Horse: 87%, Human: 100%, Mouse: 77%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against NFE2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NFE2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP31217-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFE2

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Alternative Name: NFE2

Background:

Background Information: NFE2 is a component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. NFE2 binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. NFE2 is requires MAFK or other small MAF proteins for binding to the NF-E2 motif. NFE2 may play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

Gene Name: Nuclear factor (erythroid-derived 2), 45 kDa

Alternative Symbols: p45, NF-E2

Protein Name: Transcription factor NF-E2 45 kDa subunit

Molecular Weight: 41kDa

Gene ID: 4778

NCBI Accession: NP_006154

UniProt: Q16621