NFE2 antibody (N-Term)

Catalog No. ABIN2777230

Product Details anti-NFE2 Antibody

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Horse, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFE2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MSPCPPQQSR NRVIQLSTSE LGEMELTWQE IMSITELQGL NAPSEPSFEP

Predicted Reactivity: Cow: 92%, Guinea Pig: 87%, Horse: 87%, Human: 100%, Mouse: 77%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against NFE2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NFE2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 373 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFE2

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Alternative Name: NFE2 (NFE2 Products)

Synonyms: NF-E2 antibody, p45 antibody, 45kDa antibody, p45NFE2 antibody, p45nf-e2 antibody, nuclear factor, erythroid 2 antibody, nuclear factor, erythroid derived 2 antibody, NFE2 antibody, Nfe2 antibody

Background: NFE2 is a component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. NFE2 binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. NFE2 is requires MAFK or other small MAF proteins for binding to the NF-E2 motif. NFE2 may play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
Alias Symbols: NF-E2, p45
Protein Interaction Partner: BTRC, NFE2L3, NFE2L2, NFE2, ATF4, Dlg4, TXN, ITCH, Yap1, Nedd4, WWP1, MAPK8, KAT2B, SCAND1, SSX5, HMGN1, ELF5, PHYHIP, MAFF, MAFG, PEX14, MAFK, CREBBP,
Protein Size: 373

Molecular Weight: 41 kDa

Gene ID: 4778

NCBI Accession: NM_006163, NP_006154

UniProt: Q16621