SPI1 antibody (Middle Region)

Catalog No. ABIN182360

The Rabbit Polyclonal anti-SPI1 antibody is suitable to detect SPI1 in samples from Human, Mouse, Rat, Pig, Dog, Cow, Horse and Guinea Pig. It has been validated for WB.

Quick Overview for SPI1 antibody (Middle Region) (ABIN182360)

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Reactivity: Human, Mouse, Rat, Pig, Dog, Cow, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPI1 Antibody

Binding Specificity: Middle Region

Sequence: HPSLGHQVSY LPRMCLQYPS LSPAQPSSDE EEGERQSPPL EVSDGEADGL

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 92%, Pig: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SPI1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SPI1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 264 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: SPI1

Background: SPI1 is known to collaborate with multiple transcription factors and to regulate expression of immunoglobulin genes. Lack of SPI1 expression is associated with defective immunoglobulin transcription in Hodgkin and Reed-Sternberg cells of classical Hodgkin disease
Alias Symbols: OF, PU.1, SFPI1, SPI-1, SPI-A
Protein Interaction Partner: HOXA10, TMX1, VAV1, IRF1, IRF8, GATA3, GATA2, GATA1, GFI1, TBP, RB1, BYRF1, RARA, PML, HDAC3, SKI, SIN3A, HDAC1, BCL6, CEBPA, PIP, SPIB, SSRP1, IRF4, KAT6A, CEBPE, NFATC1, CREBBP, NONO, CEBPB, JUN, ERG, NFKB1, MITF, FUS, CREM, ATF1, CEBPD, MECP2, DNMT3B,
Protein Size: 264

Molecular Weight: 30 kDa

Gene ID: 6688

NCBI Accession: NM_003120, NP_003111

UniProt: P17947

Pathways: Stem Cell Maintenance