EBF1 antibody (Early B-Cell Factor 1) (N-Term)

Details for Product anti-EBF1 Antibody No. ABIN2777379, Supplier: Log in to see
Antigen
  • EBF1
  • 9830166E18Rik
  • AW990631
  • Ebf
  • O/E-1
  • Olf-1
  • Olf1
  • COE1
  • EBF
  • OLF1
  • early B-cell factor 1
  • early B cell factor 1
  • EBF1
  • Ebf1
Epitope
N-Term
6
5
4
3
3
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
47
19
6
3
3
2
2
2
2
2
2
2
2
1
1
1
Host
Rabbit
21
18
8
1
1
Clonality
Polyclonal
Conjugate
This EBF1 antibody is un-conjugated
1
1
1
1
1
1
Application
Western Blotting (WB)
48
29
9
4
2
2
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human EBF1
Blocking Peptide Blocking peptide for this product available: ABIN976092
Sequence MCRVLLTHEI MCSRCCDKKS CGNRNETPSD PVIIDRFFLK FFLKCNQNCL
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against EBF1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Plasmids, Primers & others Plasmids, Primers & others EBF1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name EBF1 (EBF1 Antibody Abstract)
Background EBF1 belongs to the COE family. It contains 1 IPT/TIG domain. EBF1 is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
Alias Symbols: COE1, EBF, O/E-1, OLF1
Protein Interaction Partner: ATPAF2, HOMEZ, BCL6, ZNF521, ZNF423, CREBBP, PAX5, EBF1,
Protein Size: 591
Molecular Weight 64 kDa
Gene ID 1879
NCBI Accession NM_024007, NP_076870
UniProt Q9UH73
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 591 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
 image for anti-Early B-Cell Factor 1 (EBF1) (N-Term) antibody (ABIN2777379) anti-Early B-Cell Factor 1 (EBF1) (N-Term) antibody
Western Blotting (WB) image for anti-Early B-Cell Factor 1 (EBF1) (N-Term) antibody (ABIN2777379) EBF1 antibody - N-terminal region validated by WB using striatum tissue lysate
Western Blotting (WB) image for anti-Early B-Cell Factor 1 (EBF1) (N-Term) antibody (ABIN2777379) WB Suggested Anti-EBF1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Posi...
Background publications Roessler, Györy, Imhof, Spivakov, Williams, Busslinger, Fisher, Grosschedl: "Distinct promoters mediate the regulation of Ebf1 gene expression by interleukin-7 and Pax5." in: Molecular and cellular biology, Vol. 27, Issue 2, pp. 579-94, 2007 (PubMed).

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