PIAS3 antibody (Middle Region)

Catalog No. ABIN2777575

The Rabbit Polyclonal anti-PIAS3 antibody (ABIN2777575) specifically detects PIAS3 in WB. The antibody is reactive with Human, Rat, Dog, Cow, Horse, Guinea Pig and Rabbit samples.

Quick Overview for PIAS3 antibody (Middle Region) (ABIN2777575)

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Reactivity: Human, Rat, Dog, Cow, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIAS3 Antibody

Binding Specificity: Middle Region

Purpose: PIAS3 Antibody - middle region

Sequence: DEIQFMEDGS WCPMKPKKEA SEVCPPPGYG LDGLQYSPVQ GGDPSENKKK

Predicted Reactivity: Cow: 79%, Dog: 86%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Rabbit: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against PIAS3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PIAS3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32763-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PIAS3

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Alternative Name: PIAS3

Background:

Background Information: PIAS3 is a member of the protein inhibitor of activated STAT (PIAS) family. It also activates TGF-beta/SMAD transcriptional responses. This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Protein inhibitor of activated STAT, 3

Alternative Symbols: ZMIZ5

Protein Name: E3 SUMO-protein ligase PIAS3

Molecular Weight: 68kDa

Gene ID: 10401

NCBI Accession: NP_006090

UniProt: Q9Y6X2

Pathways: JAK-STAT Signaling