PCCA antibody (N-Term)

Catalog No. ABIN2777736

Cited in 1 publication. The Rabbit Polyclonal anti-PCCA antibody (ABIN2777736) specifically detects PCCA in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Horse, Zebrafish (Danio rerio), Dog, Pig and Rabbit samples.

Quick Overview for PCCA antibody (N-Term) (ABIN2777736)

Target: PCCA (Propionyl CoA Carboxylase, alpha Polypeptide (PCCA))

Reactivity: Human, Mouse, Rat, Cow, Horse, Zebrafish (Danio rerio), Dog, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCCA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PCCA Antibody

Binding Specificity: N-Term

Purpose: PCCA Antibody - N-terminal region

Sequence: LYYSRQCLMV SRNLGSVGYD PNEKTFDKIL VANRGEIACR VIRTCKKMGI

Predicted Reactivity: Cow: 86%, Dog: 86%, Horse: 83%, Human: 100%, Mouse: 77%, Pig: 86%, Rabbit: 86%, Rat: 86%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against PCCA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PCCA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8109054

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PCCA antibody (ABIN2777736)

Rincón, Aguado, Desviat, Sánchez-Alcudia, Ugarte, Pérez: "Propionic and methylmalonic acidemia: antisense therapeutics for intronic variations causing aberrantly spliced messenger RNA." in: American journal of human genetics, Vol. 81, Issue 6, pp. 1262-70, (2011) (PubMed).

Target Details for PCCA

Target: PCCA (Propionyl CoA Carboxylase, alpha Polypeptide (PCCA))

Alternative Name: PCCA

Background:

Background Information: PCCA is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA is the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia.The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: Propionyl CoA carboxylase, alpha polypeptide

Alternative Symbols: -

Protein Name: Propionyl-CoA carboxylase alpha chain, mitochondrial

Molecular Weight: 75kDa

Gene ID: 5095

NCBI Accession: NP_000273

UniProt: Q8WXQ7

Pathways: Monocarboxylic Acid Catabolic Process