ARID4A antibody (C-Term)

Catalog No. ABIN2777742

This Rabbit Polyclonal antibody specifically detects ARID4A in WB. It exhibits reactivity toward Human, Dog, Cow, Guinea Pig, Horse, Mouse, Pig, Rabbit and Ratand has been mentioned in 1 publication.

Quick Overview for ARID4A antibody (C-Term) (ABIN2777742)

Target: ARID4A (AT Rich Interactive Domain 4A (RBP1-Like) (ARID4A))

Reactivity: Human, Dog, Cow, Guinea Pig, Horse, Mouse, Pig, Rabbit, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARID4A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARID4A Antibody

Binding Specificity: C-Term

Sequence: NSSKCTPVKH LNVSKPQKLA RSPARISPHI KDGEKDKHRE KHPNSSPRTY

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 86%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against ARID4A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ARID4A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1257 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ARID4A antibody (ABIN2777742)

Kondou, Kawai, Tachikawa, Kimoto, Yamagata, Koinuma, Yamazaki, Nakayama, Mushiake, Ozono, Michigami: "Sodium-coupled neutral amino acid transporter 4 functions as a regulator of protein synthesis during liver development." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 43, Issue 11, pp. 1211-23, (2014) (PubMed).

Target Details for ARID4A

Target: ARID4A (AT Rich Interactive Domain 4A (RBP1-Like) (ARID4A))

Alternative Name: ARID4A

Background: ARID4A encodes a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein (pRB) which regulates cell proliferation. pRB represses transcription by recruiting the encoded protein. This protein, in turn, serves as a bridging molecule to recruit HDACs and, in addition, provides a second HDAC-independent repression function. The encoded protein possesses transcriptional repression activity. Multiple alternatively spliced transcripts have been observed for this gene, although not all transcript variants have been fully described.
Alias Symbols: RBP1, RBBP1, RBP-1, RBBP-1
Protein Interaction Partner: SUMO2, SUMO1, HDAC1, BRMS1, HIST2H3C, HIST4H4, UBC, CDK2, CDK1, CCNE1, CCNB1, CCNA2, CCND1, CDK4, SIN3A, SAP30, RB1, SIN3B, RBBP7, RBBP4, ARID4B, HDAC3, EID1, HDAC2, ING2, RBL1,
Protein Size: 1257

Molecular Weight: 143 kDa

Gene ID: 5926

NCBI Accession: NM_002892, NP_002883

UniProt: P29374