NFS1 antibody (Middle Region)

Catalog No. ABIN2777759

This Rabbit Polyclonal antibody specifically detects NFS1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Rabbit and Saccharomyces cerevisiae.

Quick Overview for NFS1 antibody (Middle Region) (ABIN2777759)

Target: NFS1 (NFS1, Cysteine Desulfurase (NFS1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFS1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NFS1 Antibody

Binding Specificity: Middle Region

Purpose: NFS1 Antibody - middle region

Sequence: TTQTEHKCVL DSCRSLEAEG FQVTYLPVQK SGIIDLKELE AAIQPDTSLV

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Yeast: 92%

Characteristics: This is a rabbit polyclonal antibody against NFS1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFS1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33115-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFS1

Target: NFS1 (NFS1, Cysteine Desulfurase (NFS1))

Alternative Name: NFS1

Background:

Background Information: Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH . The encoded protein belongs to the class-V family of pyridoxal phosphate-dependent aminotransferases. Two transcript variants encoding two different isoforms have been found for this gene.

Gene Name: NFS1 nitrogen fixation 1 homolog (S. cerevisiae)

Alternative Symbols: IscS, NIFS, HUSSY-08

Protein Name: Cysteine desulfurase, mitochondrial

Molecular Weight: 50kDa

Gene ID: 9054

NCBI Accession: NP_066923

UniProt: Q9Y697

Pathways: Transition Metal Ion Homeostasis