T-Box 5 antibody (N-Term)

Catalog No. ABIN2777915

There are 3+ publications for this product available. The Rabbit Polyclonal anti-T-Box 5 antibody is suitable to detect T-Box 5 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Cow, Horse, Zebrafish (Danio rerio), Goat and Sheep. It has been validated for WB and IHC.

Quick Overview for T-Box 5 antibody (N-Term) (ABIN2777915)

Target: T-Box 5 (TBX5)

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Cow, Horse, Zebrafish (Danio rerio), Goat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Box 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-T-Box 5 Antibody

Binding Specificity: N-Term

Purpose: TBX5 Antibody - N-terminal region

Sequence: NSMHKYQPRL HIVKADENNG FGSKNTAFCT HVFPETAFIA VTSYQNHKIT

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against TBX5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TBX5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8113383

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-T-Box 5 antibody (ABIN2777915)

Baban, Pitto, Pulignani, Cresci, Mariani, Gambacciani, Digilio, Pongiglione, Albanese: "Holt-Oram syndrome with intermediate atrioventricular canal defect, and aortic coarctation: functional characterization of a de novo TBX5 mutation." in: American journal of medical genetics. Part A, Vol. 164A, Issue 6, pp. 1419-24, (2014) (PubMed).

Zhang, Huang, Zuo, Chen, Wang: "Phenanthrene exposure causes cardiac arrhythmia in embryonic zebrafish via perturbing calcium handling." in: Aquatic toxicology (Amsterdam, Netherlands), Vol. 142-143, pp. 26-32, (2013) (PubMed).

Lu, Tsai, Choo, Yeh, Tang, Yang, Lee, Lu: "Induction of apoptosis and inhibition of cell growth by tbx5 knockdown contribute to dysmorphogenesis in Zebrafish embryos." in: Journal of biomedical science, Vol. 18, pp. 73, (2011) (PubMed).

Target Details for T-Box 5

Target: T-Box 5 (TBX5)

Alternative Name: TBX5

Background:

Background Information: TBX5 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. TBX5 is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in TBX5 have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for TBX5.

Gene Name: T-box 5

Alternative Symbols: HOS

Protein Name: T-box transcription factor TBX5

Molecular Weight: 58 kDa

Gene ID: 6910

NCBI Accession: NP_000183

UniProt: Q99593