TWIST1 antibody (N-Term)

Catalog No. ABIN2777932

The Rabbit Polyclonal anti-TWIST1 antibody is suitable to detect TWIST1 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow and Horse. It has been validated for WB.

Quick Overview for TWIST1 antibody (N-Term) (ABIN2777932)

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TWIST1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TWIST1 Antibody

Binding Specificity: N-Term

Purpose: TWIST1 Antibody - N-terminal region

Sequence: DSLSNSEEEP DRQQPPSGKR GGRKRRSSRR SAGGGAGPGG AAGGGVGGGD

Predicted Reactivity: Cow: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 83%

Characteristics: This is a rabbit polyclonal antibody against TWIST1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human TWIST1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33423-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TWIST1

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Alternative Name: TWIST1

Background:

Background Information: Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue, in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.

Gene Name: twist homolog 1 (Drosophila)

Alternative Symbols: CRS, CSO, SCS, ACS3, CRS1, BPES2, BPES3, SWCOS, TWIST, bHLHa38

Protein Name: Twist-related protein 1

Molecular Weight: 22kDa

Gene ID: 7291

NCBI Accession: NP_000465

UniProt: Q15672

Pathways: p53 Signaling, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity