TRIM25 antibody (C-Term)

Catalog No. ABIN2778060

The Rabbit Polyclonal anti-TRIM25 antibody is suitable to detect TRIM25 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Horse and Dog. It has been validated for WB.

Quick Overview for TRIM25 antibody (C-Term) (ABIN2778060)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM25 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TRIM25 Antibody

Binding Specificity: C-Term

Sequence: NSASWCVEWF NTKISAWHNN VEKTLPSTKA TRVGVLLNCD HGFVIFFAVA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TRIM25. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human TRIM25

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: TRIM25

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Alias Symbols: EFP, Z147, RNF147, ZNF147
Protein Size: 422

Molecular Weight: 46 kDa

Gene ID: 7706

NCBI Accession: NM_005082, NP_005073

UniProt: Q14258