PARP2 antibody (Middle Region)

Catalog No. ABIN2778112

This anti-PARP2 antibody is a Rabbit Polyclonal antibody detecting PARP2 in WB and IF. Suitable for Human, Mouse, Rat, Dog, Cow, Horse, Rabbit and Guinea Pig.

Quick Overview for PARP2 antibody (Middle Region) (ABIN2778112)

Target: PARP2 (Poly (ADP-Ribose) Polymerase 2 (PARP2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-PARP2 Antibody

Binding Specificity: Middle Region

Sequence: LLDLFEVEKD GEKEAFREDL HNRMLLWHGS RMSNWVGILS HGLRIAHPEA

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 92%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 86%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against PARP2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PARP2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 534 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PARP2

Target: PARP2 (Poly (ADP-Ribose) Polymerase 2 (PARP2))

Alternative Name: PARP2

Background: PARP2 contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of protein.
Alias Symbols: ADPRT2, PARP-2, ADPRTL2, ADPRTL3, pADPRT-2
Protein Interaction Partner: UHRF1, Npm1, TFAP4, UBC, RNF146, H3F3A, CBX5, TRIM28, CHD1L, TNP2, HSPA2, BUB3, CASP8, XRCC1, PARP1, CENPB, CENPA,
Protein Size: 534

Molecular Weight: 61 kDa

Gene ID: 10038

NCBI Accession: NM_005484, NP_005475

UniProt: Q9UGN5

Pathways: DNA Damage Repair