CDC25A antibody (N-Term)

Catalog No. ABIN2778347

This Rabbit Polyclonal antibody specifically detects CDC25A in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit and Guinea Pig.

Quick Overview for CDC25A antibody (N-Term) (ABIN2778347)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDC25A Antibody

Binding Specificity: N-Term

Purpose: CDC25A Antibody - N-terminal region

Sequence: QLQGLGSDYE QPLEVKNNSN LQRMGSSEST DSGFCLDSPG PLDSKENLEN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CDC25A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human CDC25A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP34244-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: CDC25A

Background:

Background Information: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: cell division cycle 25 homolog A (S. pombe)

Alternative Symbols: CDC25A2

Protein Name: M-phase inducer phosphatase 1

Molecular Weight: 59 kDa

Gene ID: 993

NCBI Accession: NP_001780

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase