SIRT5 antibody (Middle Region)

Catalog No. ABIN2778620

The Rabbit Polyclonal anti-SIRT5 antibody (ABIN2778620) specifically detects SIRT5 in WB and IHC. The antibody is reactive with Human, Mouse, Cow, Goat, Rabbit and Pig samples.

Quick Overview for SIRT5 antibody (Middle Region) (ABIN2778620)

Target: SIRT5 (Sirtuin 5 (SIRT5))

Reactivity: Human, Mouse, Cow, Goat, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SIRT5 Antibody

Binding Specificity: Middle Region

Sequence: PICPALSGKG APEPGTQDAS IPVEKLPRCE EAGCGGLLRP HVVWFGENLD

Predicted Reactivity: Cow: 79%, Goat: 79%, Human: 100%, Mouse: 79%, Pig: 86%, Rabbit: 86%

Characteristics: This is a rabbit polyclonal antibody against SIRT5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SIRT5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 310 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SIRT5

Target: SIRT5 (Sirtuin 5 (SIRT5))

Alternative Name: SIRT5

Background: SIRT5 is included in class III of the sirtuin family which ischaracterized by a sirtuin core domain. Human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in two transcript variants.
Alias Symbols: SIR2L5
Protein Interaction Partner: SIRT3, UBC, HECW2, APP, RELA,
Protein Size: 310

Molecular Weight: 34 kDa

Gene ID: 23408

NCBI Accession: NM_012241, NP_036373

UniProt: Q9NXA8

Pathways: SARS-CoV-2 Protein Interactome