RPL13 antibody (C-Term)

Catalog No. ABIN2778677

This Rabbit Polyclonal antibody specifically detects RPL13 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for RPL13 antibody (C-Term) (ABIN2778677)

Target: RPL13 (Ribosomal Protein L13 (RPL13))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RPL13 Antibody

Binding Specificity: C-Term

Purpose: RPL13 Antibody - C-terminal region

Sequence: KKEKARVITE EEKNFKAFAS LRMARANARL FGIRAKRAKE AAEQDVEKKK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RPL13. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RPL13

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8110618

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RPL13 antibody (ABIN2778677)

Hartl, Ni, Cao, Suyama, Patchett, Bussiere, Gui, Tang, Kaplan, Fish, Johnson, Scott: "Regulation of ribosome biogenesis by nucleostemin 3 promotes local and systemic growth in Drosophila." in: Genetics, Vol. 194, Issue 1, pp. 101-15, (2013) (PubMed).

Target Details for RPL13

Target: RPL13 (Ribosomal Protein L13 (RPL13))

Alternative Name: RPL13

Background:

Background Information: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Gene Name: ribosomal protein L13

Alternative Symbols: L13, BBC1, D16S44E, SEMDIST, D16S444E

Protein Name: 60S ribosomal protein L13

Molecular Weight: 24kDa

Gene ID: 6137

NCBI Accession: NP_000968

UniProt: P26373