RAE1 antibody (C-Term)

Catalog No. ABIN2778733

This Rabbit Polyclonal antibody specifically detects RAE1 in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Cow, Horse, Rabbit and Guinea Pig.

Quick Overview for RAE1 antibody (C-Term) (ABIN2778733)

Target: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RAE1 Antibody

Binding Specificity: C-Term

Purpose: RAE1 Antibody - C-terminal region

Sequence: EQLDQPISAC CFNHNGNIFA YASSYDWSKG HEFYNPQKKN YIFLRNAAEE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against RAE1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RAE1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40301-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RAE1

Target: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Alternative Name: RAE1

Background:

Background Information: RAE1 is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Gene Name: RAE1 RNA export 1 homolog (S. pombe)

Alternative Symbols: Gle2, MIG14, MRNP41, Mnrp41, dJ481F12.3, dJ800J21.1

Protein Name: mRNA export factor

Molecular Weight: 40kDa

Gene ID: 8480

NCBI Accession: NP_001015885

UniProt: P78406

Pathways: SARS-CoV-2 Protein Interactome