SF3B4 antibody (N-Term)

Catalog No. ABIN2778952

The Rabbit Polyclonal anti-SF3B4 antibody is suitable to detect SF3B4 in samples from Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig, Rabbit, Saccharomyces cerevisiae, Horse and Goat. It has been validated for WB and IHC.

Quick Overview for SF3B4 antibody (N-Term) (ABIN2778952)

Target: SF3B4 (Splicing Factor 3b, Subunit 4, 49kDa (SF3B4))

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig, Rabbit, Saccharomyces cerevisiae, Horse, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3B4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SF3B4 Antibody

Binding Specificity: N-Term

Purpose: SF3B4 Antibody - N-terminal region

Sequence: QDATVYVGGL DEKVSEPLLW ELFLQAGPVV NTHMPKDRVT GQHQGYGFVE

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 86%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 86%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SF3B4. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SF3B4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8112735

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SF3B4

Target: SF3B4 (Splicing Factor 3b, Subunit 4, 49kDa (SF3B4))

Alternative Name: SF3B4

Background:

Targeted Subunit: 4

Background Information: SF3B4 is one of four subunits of the splicing factor 3B. The protein cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.

Gene Name: Splicing factor 3b, subunit 4, 49 kDa

Alternative Symbols: AFD1, Hsh49, SAP49, SF3b49

Protein Name: Splicing factor 3B subunit 4

Molecular Weight: 47kDa

Gene ID: 10262

NCBI Accession: NP_005841

UniProt: Q15427