CPSF4 antibody (C-Term)

Catalog No. ABIN2779005

The Rabbit Polyclonal anti-CPSF4 antibody (ABIN2779005) specifically detects CPSF4 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow and Rabbit samples.

Quick Overview for CPSF4 antibody (C-Term) (ABIN2779005)

Target: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPSF4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CPSF4 Antibody

Binding Specificity: C-Term

Purpose: CPSF4 Antibody - C-terminal region

Sequence: SLIQLTSQNS SPNQQRTPQV IGVMQSQNSS AGNRGPRPLE QVTCYKCGEK

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CPSF4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CPSF4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8097529

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CPSF4

Target: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Alternative Name: CPSF4

Background:

Targeted Subunit: 4

Background Information: Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.

Gene Name: Cleavage and polyadenylation specific factor 4, 30 kDa

Alternative Symbols: NAR, NEB1, NEB-1, CPSF30

Protein Name: Cleavage and polyadenylation specificity factor subunit 4

Molecular Weight: 24kDa

Gene ID: 10898

UniProt: O95639