NONO antibody (C-Term)

Catalog No. ABIN2779028

This Rabbit Polyclonal antibody specifically detects NONO in WB, IHC and IF. It exhibits reactivity toward Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig and Rabbitand has been mentioned in 1 publication.

Quick Overview for NONO antibody (C-Term) (ABIN2779028)

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NONO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NONO Antibody

Binding Specificity: C-Term

Sequence: DGTLGLTPPT TERFGQAATM EGIGAIGGTP PAFNRAAPGA EFAPNKRRRY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NONO. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NONO

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 471 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NONO antibody (ABIN2779028)

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for NONO

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Alternative Name: NONO

Background: NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
Alias Symbols: P54, NMT55, NRB54, P54NRB
Protein Interaction Partner: SFPQ, PTBP1, PRKAA2, PIN1, NONO, FUS, DDX6, C11orf68, PSPC1, HUWE1, LMO4, UBC, SUMO2, STAU1, MDM2, RPA3, RPA2, RPA1, ASB18, WWOX, ASB3, ERG, EED, rev, WBP4, TCERG1, APBB1, PRPF40A, LMNA, CD81, PAN2, CTNNB1, ORC5, MYC, ITGA4, IL7R, FN1, EWSR1, YLPM1, RNF43
Protein Size: 471

Molecular Weight: 52 kDa

Gene ID: 4841

NCBI Accession: NM_007363, NP_031389

UniProt: Q15233