SF3B1 antibody (N-Term)

Catalog No. ABIN2779036

This Rabbit Polyclonal antibody specifically detects SF3B1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Goat and Zebrafish (Danio rerio).

Quick Overview for SF3B1 antibody (N-Term) (ABIN2779036)

Target: SF3B1 (Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Goat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SF3B1 Antibody

Binding Specificity: N-Term

Sequence: ERLDPFADGG KTPDPKMNAR TYMDVMREQH LTKEEREIRQ QLAEKAKAGE

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against SF3B1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SF3B1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 789 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SF3B1

Target: SF3B1 (Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))

Alternative Name: SF3B1

Background: SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.
Alias Symbols: MDS, PRP10, Hsh155, PRPF10, SAP155, SF3b155
Protein Interaction Partner: SART3, SUMO2, SUMO3, MDM2, STAU1, UBC, LGR4, WWOX, RPA3, RPA2, RPA1, ERG, EED, RNF2, EZH2, SUZ12, APBB1, PRPF40A, WBP4, TCERG1, FBXO6, RBM17, SNIP1, SF3B6, WHSC1, RPS2, RPL23A, RPL18A, RPL10, RPL3, PLRG1, SMAD9, SMAD5, SMAD1, IL7R, FN1, ESR1, CSNK2A1, EIF
Protein Size: 789

Molecular Weight: 87 kDa

Gene ID: 23451

NCBI Accession: NM_001005526

UniProt: Q7Z497

Pathways: Chromatin Binding