HSP27 antibody (C-Term)

Catalog No. ABIN2779293

There are 2+ publications for this product available. The Rabbit Polyclonal anti-HSP27 antibody is suitable to detect HSP27 in samples from Human. It has been validated for WB and IHC.

Quick Overview for HSP27 antibody (C-Term) (ABIN2779293)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HSP27 Antibody

Binding Specificity: C-Term

Purpose: HSPB1 Antibody - C-terminal region

Sequence: SLSPEGTLTV EAPMPKLATQ SNEITIPVTF ESRAQLGGPE AAKSDETAAK

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against HSPB1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HSPB1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP30177-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HSP27 antibody (ABIN2779293)

Sookoian, Castaño, Scian, San Martino, Pirola: "Heat Shock Protein 27 is down-regulated in Ballooned Hepatocytes of Patients with Nonalcoholic Steatohepatitis (NASH)." in: Scientific reports, Vol. 6, pp. 22528, (2016) (PubMed).

Narjoz, Marisa, Imbeaud, Paris, Delacroix, Beaune, De Waziers: "Genomic consequences of cytochrome P450 2C9 overexpression in human hepatoma cells." in: Chemical research in toxicology, Vol. 22, Issue 5, pp. 779-87, (2009) (PubMed).

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSPB1

Background:

Background Information: HSPB1, like the other heat shock proteins, is part of a complex system of molecular chaperones in epidermal keratinocytes.

Gene Name: Heat shock 27 kDa protein 1

Alternative Symbols: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102

Protein Name: Heat shock protein beta-1

Molecular Weight: 23kDa

Gene ID: 3315

NCBI Accession: NP_001531

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling