RAD17 antibody (C-Term)

Catalog No. ABIN2779300

Cited in 1 publication. The Rabbit Polyclonal anti-RAD17 antibody (ABIN2779300) specifically detects RAD17 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow and Pig samples.

Quick Overview for RAD17 antibody (C-Term) (ABIN2779300)

Target: RAD17

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD17 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAD17 Antibody

Binding Specificity: C-Term

Purpose: RAD17 Antibody - C-terminal region

Sequence: PTQATVPETW SLPLSQNSAS ELPASQPQPF SAQGDMEENI IIEDYESDGT

Predicted Reactivity: Cow: 83%, Dog: 75%, Human: 100%, Mouse: 75%, Pig: 83%, Rat: 75%

Characteristics: This is a rabbit polyclonal antibody against RAD17. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RAD17

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8109903

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RAD17 antibody (ABIN2779300)

Tsao, Geisen, Abraham: "Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling." in: The EMBO journal, Vol. 23, Issue 23, pp. 4660-9, (2004) (PubMed).

Target Details for RAD17

Target: RAD17

Alternative Name: RAD17

Background:

Background Information: RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.

Gene Name: RAD17 homolog (S. pombe)

Alternative Symbols: CCYC, R24L, RAD24, HRAD17, RAD17SP

Protein Name: Cell cycle checkpoint protein RAD17

Molecular Weight: 66kDa

Gene ID: 5884

NCBI Accession: NP_579919

UniProt: O75943