DFFA antibody (N-Term)

Catalog No. ABIN2779329

The Rabbit Polyclonal anti-DFFA antibody (ABIN2779329) specifically detects DFFA in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig and Horse samples.

Quick Overview for DFFA antibody (N-Term) (ABIN2779329)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DFFA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DFFA Antibody

Binding Specificity: N-Term

Purpose: DFFA Antibody - N-terminal region

Sequence: MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL

Predicted Reactivity: Cow: 92%, Dog: 83%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 83%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against DFFA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DFFA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099047

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Alternative Name: DFFA

Background:

Targeted Subunit: alpha

Background Information: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Gene Name: DNA fragmentation factor, 45 kDa, alpha polypeptide

Alternative Symbols: DFF1, ICAD, DFF-45

Protein Name: DNA fragmentation factor subunit alpha

Molecular Weight: 37kDa

Gene ID: 1676

NCBI Accession: NP_004392

UniProt: O00273

Pathways: Apoptosis, Caspase Cascade in Apoptosis