DYRK3 antibody (N-Term)

Catalog No. ABIN2779364

Cited in 1 publication. The Rabbit Polyclonal anti-DYRK3 antibody (ABIN2779364) specifically detects DYRK3 in WB and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for DYRK3 antibody (N-Term) (ABIN2779364)

Target: DYRK3 (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 3 (DYRK3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DYRK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-DYRK3 Antibody

Binding Specificity: N-Term

Purpose: DYRK3 Antibody - N-terminal region

Sequence: GDHTQHFLDG GEMKVEQLFQ EFGNRKSNTI QSDGISDSEK CSPTVSQGKS

Predicted Reactivity: Human: 100%, Mouse: 86%

Characteristics: This is a rabbit polyclonal antibody against DYRK3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DYRK3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP30648-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DYRK3 antibody (ABIN2779364)

Wippich, Bodenmiller, Trajkovska, Wanka, Aebersold, Pelkmans: "Dual specificity kinase DYRK3 couples stress granule condensation/dissolution to mTORC1 signaling." in: Cell, Vol. 152, Issue 4, pp. 791-805, (2013) (PubMed).

Target Details for DYRK3

Target: DYRK3 (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 3 (DYRK3))

Alternative Name: DYRK3

Background:

Background Information: This gene product belongs to the DYRK family of dual-specificity protein kinases that catalyze autophosphorylation on serine/threonine and tyrosine residues. The members of this family share structural similarity, however, differ in their substrate specificity, suggesting their involvement in different cellular functions. The encoded protein has been shown to autophosphorylate on tyrosine residue and catalyze phosphorylation of histones H3 and H2B in vitro. Alternatively spliced transcript variants encoding different isoforms have been identified.

Gene Name: Dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3

Alternative Symbols: RED, REDK, DYRK5, hYAK3-2

Protein Name: Dual specificity tyrosine-phosphorylation-regulated kinase 3

Molecular Weight: 66 kDa

Gene ID: 8444

NCBI Accession: NP_003573

UniProt: O43781

Pathways: Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha