SMAD5 antibody (Middle Region)

Catalog No. ABIN2779414

This Rabbit Polyclonal antibody specifically detects SMAD5 in WB. It exhibits reactivity toward Human, Mouse, Rat, Zebrafish (Danio rerio), Sheep, Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for SMAD5 antibody (Middle Region) (ABIN2779414)

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Sheep, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMAD5 Antibody

Binding Specificity: Middle Region

Sequence: YPPSPASSTY PNSPASSGPG SPFQLPADTP PPAYMPPDDQ MGQDNSQPMD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against SMAD5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SMAD5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 465 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: SMAD5

Background: SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.
Alias Symbols: DWFC, JV5-1, MADH5
Protein Interaction Partner: UBC, SUMO1, HECW2, HBG2, BCAT1, SOX5, SNRPA, SNRNP70, SFPQ, RYR2, RBM4, PTPN12, PSMD11, NEDD4, SMAD4, SMAD1, ANKRD13A, PPIL4, SOX7, SMURF2, SMURF1, MTMR10, CHMP3, CXXC5, SF3B1, U2AF2, PNKP, WWP1, NAA20, EPN1, SMAD3, SMAD2, PAX6, COPS5, HGS, EP300, ELAVL1,
Protein Size: 465

Molecular Weight: 51 kDa

Gene ID: 4090

NCBI Accession: NM_005903, NP_005894

UniProt: Q99717