SMAD5 antibody (Middle Region)

Catalog No. ABIN2779414

This Rabbit Polyclonal antibody specifically detects SMAD5 in WB. It exhibits reactivity toward Human, Rat, Mouse, Sheep, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for SMAD5 antibody (Middle Region) (ABIN2779414)

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Reactivity: Human, Rat, Mouse, Sheep, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMAD5 Antibody

Binding Specificity: Middle Region

Purpose: SMAD5 Antibody - middle region

Sequence: YPPSPASSTY PNSPASSGPG SPFQLPADTP PPAYMPPDDQ MGQDNSQPMD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against SMAD5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SMAD5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8111900

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: SMAD5

Background:

Background Information: SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.

Gene Name: SMAD family member 5

Alternative Symbols: DWFC, JV5-1, MADH5

Protein Name: Mothers against decapentaplegic homolog 5

Molecular Weight: 51kDa

Gene ID: 4090

NCBI Accession: NP_005894

UniProt: Q99717