NT5C3 antibody (Middle Region)

Catalog No. ABIN2779493

The Rabbit Polyclonal anti-NT5C3 antibody (ABIN2779493) specifically detects NT5C3 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Guinea Pig and Horse samples.

Quick Overview for NT5C3 antibody (Middle Region) (ABIN2779493)

Target: NT5C3 (5'-Nucleotidase, Cytosolic III (NT5C3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NT5C3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NT5C3 Antibody

Binding Specificity: Middle Region

Purpose: NT5C3 Antibody - middle region

Sequence: VKVVSNFMDF DETGVLKGFK GELIHVFNKH DGALRNTEYF NQLKDNSNII

Predicted Reactivity: Cow: 85%, Dog: 92%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 92%, Rabbit: 92%, Rat: 92%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against NT5C3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NT5C3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8093685

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NT5C3

Target: NT5C3 (5'-Nucleotidase, Cytosolic III (NT5C3))

Alternative Name: NT5C3

Background:

Background Information: Pyrimidine 5-prime-nucleotidase (P5N), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2). The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology. Pyrimidine 5-prime-nucleotidase (P5N, EC 3.1.3.5), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2, MIM 191720). The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: 5'-nucleotidase, cytosolic III

Alternative Symbols: p36, PN-I, POMP, PSN1, UMPH, NT5C3, P5N-1, UMPH1, hUMP1, P5'N-1, cN-III

Protein Name: Cytosolic 5'-nucleotidase 3

Molecular Weight: 38kDa

Gene ID: 51251

NCBI Accession: NP_057573

UniProt: Q9H0P0