AES antibody (Middle Region)

Catalog No. ABIN2779565

This Rabbit Polyclonal antibody specifically detects AES in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for AES antibody (Middle Region) (ABIN2779565)

Target: AES (Amino-terminal Enhancer of Split (AES))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AES antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AES Antibody

Binding Specificity: Middle Region

Sequence: HKQAEIVKRL NGICAQVLPY LSQEHQQQVL GAIERAKQVT APELNSIIRQ

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against AES. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AES

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 264 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AES

Target: AES (Amino-terminal Enhancer of Split (AES))

Alternative Name: AES

Background: AES is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes.The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: AES-1, AES-2, ESP1, GRG, GRG5, TLE5
Protein Interaction Partner: MFAP1, MBNL1, SMAD3, LMO2, LMO1, KRT31, DDX6, CRX, BYSL, BCL6, AES, AEN, CARD9, PARVG, LSM2, HOMEZ, MAGOHB, RNF31, VPS37C, C1orf109, OSGIN1, MED4, PRPF31, GORASP2, KANK2, LDOC1, MTUS2, DIP2A, NCDN, BAHD1, IKZF3, U2AF2, RBPMS, FRS3, NEK6, CALCOCO2, ABI2, T
Protein Size: 264

Molecular Weight: 29 kDa

Gene ID: 166

NCBI Accession: NM_198969, NP_945320

UniProt: Q14CJ1

Pathways: WNT Signaling, SARS-CoV-2 Protein Interactome