NPAS4 antibody (Middle Region)

Catalog No. ABIN2779719

Cited in 1 publication. The Rabbit Polyclonal anti-NPAS4 antibody (ABIN2779719) specifically detects NPAS4 in WB. The antibody is reactive with Human, Rat, Mouse, Pig, Sheep, Cow, Dog, Horse, Rabbit and Guinea Pig samples.

Quick Overview for NPAS4 antibody (Middle Region) (ABIN2779719)

Target: NPAS4 (Neuronal PAS Domain Protein 4 (NPAS4))

Reactivity: Human, Rat, Mouse, Pig, Sheep, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPAS4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NPAS4 Antibody

Binding Specificity: Middle Region

Purpose: NPAS4 Antibody - middle region

Sequence: QAHLDSPSQT FPEQLSPNPT KTYFAQEGCS FLYEKLPPSP SSPGNGDCTL

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against NPAS4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NPAS4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32556-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-NPAS4 antibody (ABIN2779719)

Pruunsild, Sepp, Orav, Koppel, Timmusk: "Identification of cis-elements and transcription factors regulating neuronal activity-dependent transcription of human BDNF gene." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 9, pp. 3295-308, (2011) (PubMed).

Target Details for NPAS4

Target: NPAS4 (Neuronal PAS Domain Protein 4 (NPAS4))

Alternative Name: NPAS4

Background:

Background Information: NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (see SIM2, MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events.NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (see SIM2, MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events (Ooe et al., 2004 [PubMed 14701734]).[supplied by OMIM].

Gene Name: Neuronal PAS domain protein 4

Alternative Symbols: NXF, Le-PAS, PASD10, bHLHe79

Protein Name: Neuronal PAS domain-containing protein 4

Molecular Weight: 87kDa

Gene ID: 266743

NCBI Accession: NP_849195

UniProt: Q8IUM7