HNF4A antibody (Middle Region)

Catalog No. ABIN2779763

The Rabbit Polyclonal anti-HNF4A antibody (ABIN2779763) specifically detects HNF4A in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Cow, Pig, Dog, Horse, Rabbit, Guinea Pig, Sheep and Zebrafish (Danio rerio) samples.

Quick Overview for HNF4A antibody (Middle Region) (ABIN2779763)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human, Rat, Mouse, Cow, Pig, Dog, Horse, Rabbit, Guinea Pig, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HNF4A Antibody

Binding Specificity: Middle Region

Purpose: HNF4A Antibody - middle region

Sequence: RGQAATPETP QPSPPGGSGS EPYKLLPGAV ATIVKPLSAI PQPTITKQEV

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 93%, Rat: 93%, Sheep: 93%, Zebrafish: 90%

Characteristics: This is a rabbit polyclonal antibody against HNF4A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HNF4A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32632-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4A

Background:

Background Information: The protein encoded by HNF4A is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression

Gene Name: Hepatocyte nuclear factor 4, alpha

Alternative Symbols: TCF, HNF4, MODY, FRTS4, MODY1, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, HNF4alpha

Protein Name: Hepatocyte nuclear factor 4-alpha

Molecular Weight: 50kDa

Gene ID: 3172

NCBI Accession: NP_787110

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process