SMAD6 antibody (N-Term)

Catalog No. ABIN2779804

The Rabbit Polyclonal anti-SMAD6 antibody has been validated for WB and IHC. It is suitable to detect SMAD6 in samples from Human, Mouse, Rat, Cow, Dog, Pig, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for SMAD6 antibody (N-Term) (ABIN2779804)

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SMAD6 Antibody

Binding Specificity: N-Term

Sequence: APRDASDPLA GAALEPAGGG RSREARSRLL LLEQELKTVT YSLLKR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 91%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against SMAD6. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD6

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 496 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMAD6

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Alternative Name: SMAD6

Background: SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members, has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.
Alias Symbols: MADH6, MADH7, HsT17432
Protein Interaction Partner: ITCH, SMURF2, BPIFA1, NEDD4L, WWP2, WWP1, USP15, USP11, PIGR, NASP, LYZ, KPNA2, CTBP2, CTBP1, UBC, ACVR1, UBE2O, BAMBI, STAMBP, ACTR1B, TGFBR1, MYD88, SMAD1, BMPR2, BMPR1B, BMPR1A, ACVR2B, ACVR1B, SMURF1, PELI1, CUL2, ELAVL1, SMAD5, RPS6KA5, TSC22D1, MAPK
Protein Size: 496

Molecular Weight: 53 kDa

Gene ID: 4091

NCBI Accession: NM_005585, NP_005576

UniProt: O43541

Pathways: Chromatin Binding