Mesp2 antibody (C-Term)

Catalog No. ABIN2779870

This Rabbit Polyclonal antibody specifically detects Mesp2 in WB. It exhibits reactivity toward Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for Mesp2 antibody (C-Term) (ABIN2779870)

Target: Mesp2 (Mesoderm Posterior 2 Homolog (Mesp2))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mesp2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Mesp2 Antibody

Binding Specificity: C-Term

Purpose: MESP2 Antibody - C-terminal region

Sequence: SCQRLQPQPQ PQPQWGCWGH DAEVLSTSED QGSSPALQLP VASPTPSSGL

Predicted Reactivity: Mouse: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against MESP2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of mouse MESP2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8105203

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Mesp2 antibody (ABIN2779870)

Leschik, Stefanovic, Brinon, Pucéat: "Cardiac commitment of primate embryonic stem cells." in: Nature protocols, Vol. 3, Issue 9, pp. 1381-7, (2008) (PubMed).

Target Details for Mesp2

Target: Mesp2 (Mesoderm Posterior 2 Homolog (Mesp2))

Alternative Name: MESP2

Background:

Background Information: Somite boundaries formed during embryogenesis, at the interface between Notch1-activated and -repressed domains. Genetic and biochemical studies indicate that this interface is generated by suppression of Notch activity by mesoderm posterior 2 (Mesp2) through induction of the lunatic fringe gene (Lfng). It is proposed that the oscillation of Notch activity is arrested and translated in the wavefront by Mesp2.

Gene Name: Mesoderm posterior 2

Alternative Symbols: bHLHc, bHLHc6

Protein Name: Mesoderm posterior protein 2

Molecular Weight: 41kDa

Gene ID: 17293

NCBI Accession: NP_032615

UniProt: O08574