HIRA antibody (Middle Region)

Catalog No. ABIN2779934

The Rabbit Polyclonal anti-HIRA antibody is suitable to detect HIRA in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse and Rabbit. It has been validated for WB.

Quick Overview for HIRA antibody (Middle Region) (ABIN2779934)

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIRA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HIRA Antibody

Binding Specificity: Middle Region

Purpose: Hira Antibody - middle region

Sequence: LRSRELESSS DSDEKVHLAK PSSLSKRKLE LEVETVEKKK KGRPRKDSRL

Predicted Reactivity: Dog: 79%, Guinea Pig: 93%, Horse: 79%, Human: 86%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Hira. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of mouse Hira

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP37054-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HIRA

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Alternative Name: Hira

Background:

Background Information: Hira is required for the periodic repression of histone gene transcription during the cell cycle.Hira cooperates with ASF1A to promote replication-independent chromatin assembly.Hira is required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Gene Name: Histone cell cycle regulation defective homolog A (S. cerevisiae)

Alternative Symbols: Tupl, N28177, Tuple1, Gm15797, AA138857, D16Ertd95, D16Ertd95e

Protein Name: Protein HIRA

Molecular Weight: 112

Gene ID: 15260

NCBI Accession: NP_034565

UniProt: Q61666

Pathways: Chromatin Binding