Glypican 3 antibody (Middle Region)

Catalog No. ABIN2780199

The Rabbit Polyclonal anti-Glypican 3 antibody has been validated for WB and IHC. It is suitable to detect Glypican 3 in samples from Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse and Rabbit. There is 1 publication available.

Quick Overview for Glypican 3 antibody (Middle Region) (ABIN2780199)

Target: Glypican 3 (GPC3)

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Glypican 3 Antibody

Binding Specificity: Middle Region

Sequence: FSTIHDSIQY VQKNAGKLTT TIGKLCAHSQ QRQYRSAYYP EDLFIDKKVL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GPC3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GPC3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 580 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Glypican 3 antibody (ABIN2780199)

Bhave, Mars, Donthamsetty, Zhang, Tan, Luo, Bowen, Michalopoulos: "Regulation of liver growth by glypican 3, CD81, hedgehog, and Hhex." in: The American journal of pathology, Vol. 183, Issue 1, pp. 153-9, (2013) (PubMed).

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: GPC3

Background: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome.
Alias Symbols: SGB, DGSX, MXR7, SDYS, SGBS, OCI-5, SGBS1, GTR2-2
Protein Interaction Partner: UBC, WNT3A, WNT7B, IGF2, FGF2,
Protein Size: 580

Molecular Weight: 64 kDa

Gene ID: 2719

NCBI Accession: NM_004484, NP_004475

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process