ERCC3 antibody (N-Term)

Catalog No. ABIN2780326

There is 1 publication for this product available. The Rabbit Polyclonal anti-ERCC3 antibody is suitable to detect ERCC3 in samples from Human, Rat, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Pig and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for ERCC3 antibody (N-Term) (ABIN2780326)

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Reactivity: Human, Rat, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERCC3 Antibody

Binding Specificity: N-Term

Purpose: ERCC3 Antibody - N-terminal region

Sequence: MGKRDRADRD KKKSRKRHYE DEEDDEEDAP GNDPQEAVPS AAGKQVDESG

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 79%, Human: 100%, Pig: 93%, Rabbit: 79%, Rat: 79%, Yeast: 90%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ERCC3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ERCC3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP37963-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ERCC3 antibody (ABIN2780326)

Vijai, Topka, Villano, Ravichandran, Maxwell, Maria, Thomas, Gaddam, Lincoln, Kazzaz, Wenz, Carmi, Schrader, Hart, Lipkin, Neuhausen, Walsh, Zhang, Lejbkowicz, Rennert, Stadler, Robson, Weitzel et al.: "A Recurrent ERCC3 Truncating Mutation Confers Moderate Risk for Breast Cancer. ..." in: Cancer discovery, Vol. 6, Issue 11, pp. 1267-1275, (2016) (PubMed).

Target Details for ERCC3

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Alternative Name: ERCC3

Background:

Background Information: ERCC3 is an ATP-dependent DNA helicase that functions in nucleotide excision repair and complements xeroderma pigmentosum group B mutations. It also is the 89 kDa subunit of basal transcription factor 2 (TFIIH) and thus functions in class II transcription.

Gene Name: Excision repair cross-complementing rodent repair deficiency, complementation group 3

Alternative Symbols: XPB, BTF2, Ssl2, TTD2, GTF2H, RAD25, TFIIH

Protein Name: TFIIH basal transcription factor complex helicase XPB subunit

Molecular Weight: 89kDa

Gene ID: 2071

NCBI Accession: NP_000113

UniProt: P19447

Pathways: DNA Damage Repair