EZH1 antibody (N-Term)

Catalog No. ABIN2780390

The Rabbit Polyclonal anti-EZH1 antibody (ABIN2780390) specifically detects EZH1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for EZH1 antibody (N-Term) (ABIN2780390)

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EZH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EZH1 Antibody

Binding Specificity: N-Term

Purpose: EZH1 Antibody - N-terminal region

Sequence: MEIPNPPTSK CITYWKRKVK SEYMRLRQLK RLQANMGAKA LYVANFAKVQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against EZH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EZH1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38092-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EZH1

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Alternative Name: EZH1

Background:

Background Information: EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity.

Gene Name: Enhancer of zeste homolog 1 (Drosophila)

Alternative Symbols: KMT6B

Protein Name: Histone-lysine N-methyltransferase EZH1

Molecular Weight: 85kDa

Gene ID: 2145

NCBI Accession: NP_001982

UniProt: Q92800