JunB antibody (N-Term)

Catalog No. ABIN2780413

The Rabbit Polyclonal anti-JunB antibody has been validated for WB and IHC. It is suitable to detect JunB in samples from Human, Mouse, Rat, Cow, Dog, Horse and Guinea Pig. There is 1 publication available.

Quick Overview for JunB antibody (N-Term) (ABIN2780413)

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JunB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-JunB Antibody

Binding Specificity: N-Term

Sequence: YGRAPGGLSL HDYKLLKPSL AVNLADPYRS LKAPGARGPG PEGGGGGSYF

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against JUNB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human JUNB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 347 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-JunB antibody (ABIN2780413)

Trøen, Nygaard, Jenssen, Ikonomou, Tierens, Matutes, Gruszka-Westwood, Catovsky, Myklebost, Lauritzsen, Hovig, Delabie: "Constitutive expression of the AP-1 transcription factors c-jun, junD, junB, and c-fos and the marginal zone B-cell transcription factor Notch2 in splenic marginal zone lymphoma." in: The Journal of molecular diagnostics : JMD, Vol. 6, Issue 4, pp. 297-307, (2004) (PubMed).

Target Details for JunB

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Alternative Name: JUNB

Background: The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein, along with c-Fos, is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Jun B and Jun D, have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. JunB is involved in many types of human carcinoma including T-cell lymphomas, CML,primary cutaneous lymphomas. Aberrantly expressed c-Jun and JunB are a hallmark of Hodgkin lymphoma cells, stimulate proliferation and synergize with NF-kappa B. JunB potentiates function of BRCA1 activation domain 1 (AD1) through a coiled-coil-mediated interaction.JunB is an important regulator of erythroid Differentiation.
Alias Symbols: AP-1
Protein Interaction Partner: BATF, FOS, RFWD2, C19orf68, USP24, TCERG1, ZNF595, PKIA, MAP2, APLP2, FOSL1, DDIT3, ATF3, BATF2, BATF3, TCL1A, FOSL2, NPM1, NEU1, SMAD4, HOXA7, BDNF, ATF4, MAPK6, UBC, TDG, SET, SAT1, MAPK9, BRCA1, SUMO2, Cebpb, EP300, SMURF1, YY1, CREBBP, SMARCA4, JDP2,
Protein Size: 347

Molecular Weight: 36 kDa

Gene ID: 3726

NCBI Accession: NM_002229, NP_002220

UniProt: P17275