Phospholipase D2 antibody (N-Term)

Catalog No. ABIN2780445

The Rabbit Polyclonal anti-Phospholipase D2 antibody is suitable to detect Phospholipase D2 in samples from Human, Rat, Mouse, Cow, Horse, Dog, Guinea Pig and Rabbit. It has been validated for WB.

Quick Overview for Phospholipase D2 antibody (N-Term) (ABIN2780445)

Target: Phospholipase D2 (PLD2)

Reactivity: Human, Rat, Mouse, Cow, Horse, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase D2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Phospholipase D2 Antibody

Binding Specificity: N-Term

Purpose: PLD2 Antibody - N-terminal region

Sequence: MTATPESLFP TGDELDSSQL QMESDEVDTL KEGEDPADRM HPFLAIYELQ

Predicted Reactivity: Cow: 86%, Dog: 85%, Guinea Pig: 83%, Horse: 86%, Human: 100%, Mouse: 75%, Rabbit: 86%, Rat: 75%

Characteristics: This is a rabbit polyclonal antibody against PLD2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PLD2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38182-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Phospholipase D2

Target: Phospholipase D2 (PLD2)

Alternative Name: PLD2

Background:

Background Information: Phosphatidylcholine (PC)-specific phospholipases D (PLDs, EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.Phosphatidylcholine (PC)-specific phospholipases D (PLDs, EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-14 AF033850.1 61-74 15-614 BC015033.1 1-600 615-1279 BC056871.1 549-1213 1280-1830 BC015033.1 1266-1816 1831-1995 BC056871.1 1765-1929 1996-3489 BC015033.1 1982-3475

Gene Name: Phospholipase D2

Alternative Symbols: PLD1C

Protein Name: Phospholipase D2

Molecular Weight: 106kDa

Gene ID: 5338

NCBI Accession: NP_002654

UniProt: O14939

Pathways: RTK Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling