STAT3 antibody (N-Term)

Catalog No. ABIN2780479

Product Details anti-STAT3 Antibody

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Sequence: AQWNQLQQLD TRYLEQLHQL YSDSFPMELR QFLAPWIESQ DWAYAASKES

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against STAT3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human STAT3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 769 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-STAT3 antibody (ABIN2780479)

Deng, Chen, Li, Yuan, Li, Ke, Wang, Wu, Luo: "Bone marrow mesenchymal stromal cells with CD47 high expression via the signal transducer and activators of transcription signaling pathway preventing myocardial fibrosis." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10555-64, (2015) (PubMed).

Vega, Crotty Alexander, Charles, Hwang, Nizet, De Maio: "Activation of the stress response in macrophages alters the M1/M2 balance by enhancing bacterial killing and IL-10 expression." in: Journal of molecular medicine (Berlin, Germany), Vol. 92, Issue 12, pp. 1305-17, (2014) (PubMed).

Deng, Chen, Li, Liu, Niu, Zhou, Li, Ke, Mo et al.: "Bone marrow mesenchymal stromal cells with support of bispecific antibody and ultrasound-mediated microbubbles prevent myocardial fibrosis via the signal transducer and activators of transcription ..." in: Cytotherapy, Vol. 13, Issue 4, pp. 431-40, (2011) (PubMed).

Target Details for STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name: STAT3 (STAT3 Products)

Background: STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
Alias Symbols: APRF, FLJ20882, MGC16063, HIES
Protein Interaction Partner: MTOR, GADD45A, BMX, BLK, UBC, SYK, LMO2, SRI, HIC1, GNB2, USP22, RELA, COPS5, BRD4, ADRB2, PSMG3, TBCD, TBC1D15, PUS7, LUC7L2, ARFIP1, IVNS1ABP, MSH2, BMH1, SIN3A, EGFR, PIAS3, HDAC3, SRC, LYN, JAK3, JAK2, HES5, TRIM28, HES1, HCK, FYN, FGR, HNRNPM, SULT2A
Protein Size: 769

Molecular Weight: 88 kDa

Gene ID: 6774

NCBI Accession: NM_003150, NP_003141

UniProt: B5BTZ6

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor