TCF3 antibody (N-Term)

Catalog No. ABIN2780485

The Rabbit Polyclonal anti-TCF3 antibody is suitable to detect TCF3 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Cow and Rabbit. It has been validated for WB.

Quick Overview for TCF3 antibody (N-Term) (ABIN2780485)

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TCF3 Antibody

Binding Specificity: N-Term

Sequence: MNQPQRMAPV GTDKELSDLL DFSMMFPLPV TNGKGRPASL AGAQFGGSGL

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 77%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TCF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TCF3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 654 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TCF3

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Alternative Name: TCF3

Background: TCF3 contains 1 basic helix-loop-helix (bHLH) domain. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. TCF3 binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
Alias Symbols: E2A, E47, ITF1, VDIR, TCF-3, bHLHb21
Protein Interaction Partner: ID3, FAM115A, TLE1, RNF14, UBC, SCX, MAPKAPK2, MAPKAPK3, TCF21, Ube2i, ID2, FUS, CTNNB1, RPL37, MAX, USF1, TCF12, TCF3, MYOD1, Rufy1, Tfap4, Rpa1, Tbx6, Ncl, Twist2, SUPT3H, TRRAP, KAT2A, TADA2A, FOXH1, ELAVL1, TCF24, SETSIP, FLG2, BHLHA15, MYL6B, TIMM50,
Protein Size: 654

Molecular Weight: 68 kDa

Gene ID: 6929

NCBI Accession: NM_003200, NP_003191

UniProt: P15923

Pathways: WNT Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response