SAP30 antibody (N-Term)

Catalog No. ABIN2780555

This Rabbit Polyclonal antibody specifically detects SAP30 in WB. It exhibits reactivity toward Human, Mouse, Rabbit, Cow, Dog, Rat and Guinea Pig.

Quick Overview for SAP30 antibody (N-Term) (ABIN2780555)

Target: SAP30 (Sin3A-Associated Protein, 30kDa (SAP30))

Reactivity: Human, Mouse, Rabbit, Cow, Dog, Rat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAP30 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SAP30 Antibody

Binding Specificity: N-Term

Purpose: SAP30 Antibody - N-terminal region

Sequence: MNGFTPDEMS RGGDAAAAVA AVVAAAAAAA SAGNGTGAGT GAEVPGAGAV

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against SAP30. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SAP30

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8111775

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SAP30

Target: SAP30 (Sin3A-Associated Protein, 30kDa (SAP30))

Alternative Name: SAP30

Background:

Targeted Subunit: SAP30

Background Information: Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. SAP30 is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3.

Gene Name: Sin3A-associated protein, 30 kDa

Protein Name: Histone deacetylase complex subunit SAP30

Molecular Weight: 23kDa

Gene ID: 8819

NCBI Accession: NP_003855

UniProt: O75446