EZH2 antibody (N-Term)

Catalog No. ABIN2780595

This Rabbit Polyclonal antibody specifically detects EZH2 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig and Horse.

Quick Overview for EZH2 antibody (N-Term) (ABIN2780595)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EZH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EZH2 Antibody

Binding Specificity: N-Term

Purpose: EZH2 Antibody - N-terminal region

Sequence: KGPVCWRKRV KSEYMRLRQL KRFRRADEVK SMFSSNRQKI LERTEILNQE

Specificity: From peptide immunogen, will recognize all 5 isoforms.

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EZH2. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EZH2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38470-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2

Background:

Background Information: EZH2 is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems.

Gene Name: Enhancer of zeste homolog 2 (Drosophila)

Alternative Symbols: WVS, ENX1, KMT6, WVS2, ENX-1, EZH2b, KMT6A

Protein Name: Histone-lysine N-methyltransferase EZH2

Molecular Weight: 85kDa

Gene ID: 2146

NCBI Accession: NP_004447

UniProt: Q15910

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation