FGD1 antibody (N-Term)

Catalog No. ABIN2780596

Cited in 1 publication. The Rabbit Polyclonal anti-FGD1 antibody (ABIN2780596) specifically detects FGD1 in WB. The antibody is reactive with Human, Cow, Pig and Rat samples.

Quick Overview for FGD1 antibody (N-Term) (ABIN2780596)

Target: FGD1 (FYVE, RhoGEF and PH Domain Containing 1 (FGD1))

Reactivity: Human, Cow, Pig, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FGD1 Antibody

Binding Specificity: N-Term

Purpose: FGD1 Antibody - N-terminal region

Sequence: HGHRAPGGAG PSEPEHPATN PPGAAPPACA DSDPGASEPG LLARRGSGSA

Predicted Reactivity: Cow: 86%, Human: 100%, Pig: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against FGD1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FGD1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38472-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-FGD1 antibody (ABIN2780596)

Zou, Greenblatt, Shim, Kant, Zhai, Lotinun, Brady, Hu, Gygi, Baron, Davis, Jones, Glimcher: "MLK3 regulates bone development downstream of the faciogenital dysplasia protein FGD1 in mice." in: The Journal of clinical investigation, Vol. 121, Issue 11, pp. 4383-92, (2011) (PubMed).

Target Details for FGD1

Target: FGD1 (FYVE, RhoGEF and PH Domain Containing 1 (FGD1))

Alternative Name: FGD1

Background:

Background Information: FGD1 contains Dbl (DH) and pleckstrin (PH) homology domains. It can bind specifically to the Rho family GTPase Cdc42Hs and stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. FGD1 has an essential role in embryonic development, and FGD1 gene mutations result in the human developmental disorder, Aarskog-Scott syndrome.FGD1 contains Dbl (DH) and pleckstrin (PH) homology domains. It can bind specifically to the Rho family GTPase Cdc42Hs and stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. FGD1 has an essential role in embryonic development, and FGD1 gene mutations result in the human developmental disorder, Aarskog-Scott syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: FYVE, RhoGEF and PH domain containing 1

Alternative Symbols: AAS, FGDY, MRXS16, ZFYVE3

Protein Name: FYVE, RhoGEF and PH domain-containing protein 1

Molecular Weight: 106kDa

Gene ID: 2245

NCBI Accession: NP_004454

UniProt: P98174

Pathways: Neurotrophin Signaling Pathway