POU4F2 antibody (N-Term)

Catalog No. ABIN2780610

There is 1 publication for this product available. The Rabbit Polyclonal anti-POU4F2 antibody is suitable to detect POU4F2 in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig and Horse. It has been validated for WB.

Quick Overview for POU4F2 antibody (N-Term) (ABIN2780610)

Target: POU4F2 (POU Domain, Class 4, Transcription Factor 2 (POU4F2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU4F2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POU4F2 Antibody

Binding Specificity: N-Term

Purpose: POU4F2 Antibody - N-terminal region

Sequence: MMMMSLNSKQ AFSMPHGGSL HVEPKYSALH STSPGSSAPI APSASSPSSS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against POU4F2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POU4F2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8108773

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-POU4F2 antibody (ABIN2780610)

Tucker, Anfinson, Mullins, Stone, Young: "Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation." in: Stem cells translational medicine, Vol. 2, Issue 1, pp. 16-24, (2013) (PubMed).

Target Details for POU4F2

Target: POU4F2 (POU Domain, Class 4, Transcription Factor 2 (POU4F2))

Alternative Name: POU4F2

Background:

Background Information: POU4F2 is a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. A class IV POU-domain protein, POU4F2 is found in human retina exclusively within a subpopulation of ganglion cells where it may play a role in determining or maintaining the identities of a small subset of visual system neurons.POU4F2 is a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. A class IV POU-domain protein, POU4F2 is found in human retina exclusively within a subpopulation of ganglion cells where it may play a role in determining or maintaining the identities of a small subset of visual system neurons.[supplied by OMIM].

Gene Name: POU class 4 homeobox 2

Alternative Symbols: BRN3B, BRN3.2, Brn-3b

Protein Name: POU domain, class 4, transcription factor 2

Molecular Weight: 43 kDa

Gene ID: 5458

NCBI Accession: NP_004566

UniProt: Q12837

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Sensory Perception of Sound