NR1D2 antibody (N-Term)

Catalog No. ABIN2780649

This Rabbit Polyclonal antibody specifically detects NR1D2 in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog, Rabbit, Cow, Horse and Guinea Pig.

Quick Overview for NR1D2 antibody (N-Term) (ABIN2780649)

Target: NR1D2 (Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2))

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Cow, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR1D2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NR1D2 Antibody

Binding Specificity: N-Term

Sequence: YISSSSSASS HASCHSEGSE NSFQSSSSSV PSSPNSSNSD TNGNPKNGDL

Predicted Reactivity: Cow: 79%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against NR1D2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NR1D2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 579 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NR1D2

Target: NR1D2 (Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2))

Alternative Name: NR1D2

Background: NR1D2 can interact with NCOA5 coactivator, leading to a strong increase of transcription of target genes. NR1D2 also binds to the sequences 5'-AATGTAGGTCA-3' and 5'-ATAACTAGGTCA-3' and acts as a potent competitive transcriptional silencer and negative regulator of RORalpha mediated trans-activation. NR1D2 may play different roles in metabolism, inflammation, and circadian cycling in the organ-specific manner in homeostasis.
Alias Symbols: BD73, EAR-1r, HZF2, Hs.37288, RVR, EAR-1R
Protein Interaction Partner: MID2, RBPMS, TRIM27, NOTCH2NL, KRTAP10-9, KRT40, KRTAP4-2, PRMT6, SKP1, UBC, SIN3B, KAT5, NCOR1, TAF9, TAF6, HDAC1, GTF2B, NCOA5, UBE2I, CUL1, NR1D1, RGN,
Protein Size: 579

Molecular Weight: 65 kDa

Gene ID: 9975

NCBI Accession: NM_005126, NP_005117

UniProt: Q14995

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway