SMAD4 antibody (N-Term)

Catalog No. ABIN2780673

This Rabbit Polyclonal antibody specifically detects SMAD4 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Goat, Guinea Pig, Horse, Rabbit and Sheep.

Quick Overview for SMAD4 antibody (N-Term) (ABIN2780673)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SMAD4 Antibody

Binding Specificity: N-Term

Purpose: SMAD4 Antibody - N-terminal region

Sequence: MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SMAD4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38605-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background:

Background Information: SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantl

Gene Name: SMAD family member 4

Alternative Symbols: JIP, DPC4, MADH4, MYHRS

Protein Name: Mothers against decapentaplegic homolog 4

Molecular Weight: 60kDa

Gene ID: 4089

NCBI Accession: NP_005350

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy