SMAD4 antibody (C-Term)

Catalog No. ABIN2780674

The Rabbit Polyclonal anti-SMAD4 antibody is suitable to detect SMAD4 in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Goat, Guinea Pig, Horse, Rabbit and Sheep. It has been validated for WB.

Quick Overview for SMAD4 antibody (C-Term) (ABIN2780674)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMAD4 Antibody

Binding Specificity: C-Term

Sequence: AAAGIGVDDL RRLCILRMSF VKGWGPDYPR QSIKETPCWI EIHLHRALQL

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against Smad4. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of mouse SMAD4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: Smad4

Background: This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
Alias Symbols: JIP, DPC4, MADH4, MYHRS
Protein Size: 455

Molecular Weight: 50 kDa

Gene ID: 4089

NCBI Accession: NM_005359, NP_005350

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy