SMAD2 antibody (N-Term)

Catalog No. ABIN2780721

The Rabbit Polyclonal anti-SMAD2 antibody is suitable to detect SMAD2 in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Sheep, Dog, Horse and Guinea Pig. It has been validated for WB.

Quick Overview for SMAD2 antibody (N-Term) (ABIN2780721)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Sheep, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMAD2 Antibody

Binding Specificity: N-Term

Purpose: SMAD2 Antibody - N-terminal region

Sequence: VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK KLKKTGRLDE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SMAD2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8111896

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: SMAD2

Background:

Background Information: SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.

Gene Name: SMAD family member 2

Alternative Symbols: JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2

Protein Name: Mothers against decapentaplegic homolog 2

Molecular Weight: 51kDa

Gene ID: 4087

NCBI Accession: NP_005892

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus