TARDBP antibody (N-Term)

Catalog No. ABIN2780848

The Rabbit Polyclonal anti-TARDBP antibody has been validated for WB and IHC. It is suitable to detect TARDBP in samples from Human, Mouse, Rat, Dog, Cow, Horse and Guinea Pig. There is 1 publication available.

Quick Overview for TARDBP antibody (N-Term) (ABIN2780848)

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TARDBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TARDBP Antibody

Binding Specificity: N-Term

Sequence: MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TARDBP. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TARDBP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 414 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TARDBP antibody (ABIN2780848)

Estes, Boehringer, Zwick, Tang, Grigsby, Zarnescu: "Wild-type and A315T mutant TDP-43 exert differential neurotoxicity in a Drosophila model of ALS." in: Human molecular genetics, Vol. 20, Issue 12, pp. 2308-21, (2011) (PubMed).

Target Details for TARDBP

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Alternative Name: TARDBP

Background: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
Alias Symbols: ALS10, TDP-43
Protein Interaction Partner: ZFAND2B, TARDBP, UBC, SUMO2, STAU1, SUMO3, RNF4, USP8, RBM45, RNF2, LSM6, UBE2E3, GNB2L1, MED6, RPA1, WWOX, RPA3, RPA2, PARK2, EED, BMI1, RBM39, BAG2, RPL23, DDX21, PRPF3, USP10, SART1, RPL14, H1FX, FUBP3, PABPC4, EIF4G3, EIF3I, EIF3H, EIF3C, EIF3B, EIF3A
Protein Size: 414

Molecular Weight: 45 kDa

Gene ID: 23435

NCBI Accession: NM_007375, NP_031401

UniProt: Q13148

Pathways: Positive Regulation of Peptide Hormone Secretion