AATF antibody (N-Term)

Catalog No. ABIN2780859

Product Details anti-AATF Antibody

Target: AATF (Apoptosis Antagonizing Transcription Factor (AATF))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Guinea Pig, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AATF antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MAGPQPLALQ LEQLLNPRPS EADPEADPEE ATAARVIDRF DEGEDGEGDF

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against AATF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AATF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 560 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AATF

Target: AATF (Apoptosis Antagonizing Transcription Factor (AATF))

Alternative Name: AATF (AATF Products)

Background: The AATF is a protein that was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. AATF contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of AATF interfered with MAP3K12 induced apoptosis.The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.
Alias Symbols: DED, CHE1, CHE-1
Protein Interaction Partner: MDM2, DAPK3, AR, HIPK2, NGDN, UBC, TSG101, LIN28A, MMS19, ZNF468, NEDD4L, WHSC1, SMAD3, EFNB3, CPD, UBD, CAND1, PIN1, SIRT7, SUMO2, CHEK2, RELA, tat, MAGED1, PCBD2, SS18L1, LZTR1, MPHOSPH6, POLR2J, MPP6, PAWR, DAZAP2, ATR, ATM, CHEK1, LDOC1, SP1, RB1, MAP
Protein Size: 560

Molecular Weight: 63 kDa

Gene ID: 26574

NCBI Accession: NM_012138, NP_036270

UniProt: Q9NY61

Pathways: Neurotrophin Signaling Pathway, SARS-CoV-2 Protein Interactome